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  • Title: Off-line elimination of carbohydrates for amino acid analysis of samples with high carbohydrate content by ion-exchange chromatography.
    Author: Ding Y, Yu H, Mou S.
    Journal: J Chromatogr A; 2003 May 16; 997(1-2):155-60. PubMed ID: 12830888.
    Abstract:
    This paper introduces a new off-line sample preparation that eliminates carbohydrates from amino acid samples containing a high carbohydrate content before analysis by anion-exchange chromatography and integrated pulsed amperometric detection. First, the sample is introduced into a cation-exchange column in the hydrogen form. Carbohydrates are removed completely using 0.02% formic acid as a transfer fluid, while only amino acids are retained. Amino acids are then extracted from the cation-exchange resin by 10 ml of 1 M ammonia. The ammonia collected is evaporated to dryness and the residue redissolved in water containing 20 mg/l NaN3 for injection. All amino acids are recovered following the carbohydrate removal step. The average recovery is 97.2%. The relative standard deviation for seven replicates is less than 5.2%. The usefulness of the method is illustrated with chromatograms of ratafia samples obtained before and after the off-line removal of carbohydrates.
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