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  • Title: A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of recombinant expression products.
    Author: Slavícková A, Dráber P, Dráberová E, Dráber P, Viklický V.
    Journal: Folia Biol (Praha); 1992; 38(6):350-7. PubMed ID: 1284241.
    Abstract:
    A panel of seven mouse monoclonal antibodies (BG-O1 - BG-07) raised against beta-galactosidase (beta-gal) from E. coli was characterized in respect of their binding to beta-gal and to fusion proteins. The antibodies were beta-gal specific, recognized six different antigenic determinants on beta-gal molecule and some of them inhibited catalytical activity of the enzyme. The antibodies reacted with C-terminal beta-gal fusion proteins in the native state as well as after Western blotting. BG-02 antibody was successfully used for immunofluorescence detection of cells transfected with vectors containing the lacZ gene.
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