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  • Title: VLDL induces adipocyte differentiation in ApoE-dependent manner.
    Author: Chiba T, Nakazawa T, Yui K, Kaneko E, Shimokado K.
    Journal: Arterioscler Thromb Vasc Biol; 2003 Aug 01; 23(8):1423-9. PubMed ID: 12842848.
    Abstract:
    OBJECTIVE: To clarify the role of very low density lipoprotein (VLDL) and apolipoprotein E (apoE) in adipogenesis, we studied newly developed hyperlipidemic obese (ob/ob;apoE-/-) mice. Because hydrolysis of VLDL is believed to be the major source of adipogenic free fatty acids, a higher plasma level of VLDL in these mice should exaggerate obesity. METHODS AND RESULTS: When fed a high-fat, high-cholesterol diet, ob/ob;apoE-/- mice did not show increased body weight or an increased amount of adipose tissue in spite of increased plasma VLDL levels, whereas ob/ob mice showed an increased body weight and amount of adipose tissue, suggesting that there is a novel apoE-dependent pathway for adipogenesis. In vitro experiments using bone marrow stromal cells and 3T3-L1 cells confirmed this notion. ApoE-deficient VLDL did not induce adipogenesis, whereas normal VLDL induced adipogenesis in these cells. The incubation of apoE-deficient VLDL with recombinant human apoE restored its adipogenic activity. Tetrahydrolipstatin, a lipoprotein lipase inhibitor, did not affect the adipogenic activity of VLDL, suggesting that hydrolysis of VLDL did not play a major role in its effects. In fact, lipid components of VLDL or free fatty acids induced only partial adipogenesis. CONCLUSIONS: Our findings indicate that VLDL induces adipogenesis in an apoE-dependent manner both in vitro and in vivo.
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