These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors.
    Author: Vasilakis N, Falvey D, Gangolli SS, Coleman J, Kowalski J, Udem SA, Zamb TJ, Kovacs GR.
    Journal: Nat Biotechnol; 2003 Aug; 21(8):932-5. PubMed ID: 12845329.
    Abstract:
    This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use.
    [Abstract] [Full Text] [Related] [New Search]