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  • Title: Development of an inducible gene expression system for Lactobacillus sakei.
    Author: Axelsson L, Lindstad G, Naterstad K.
    Journal: Lett Appl Microbiol; 2003; 37(2):115-20. PubMed ID: 12859652.
    Abstract:
    AIM: To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production. METHODS AND RESULTS: A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters. CONCLUSION: The pKRV3 system can be used as an inducible gene expression system in lactobacilli. SIGNIFICANCE AND IMPACT OF THE STUDY: A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.
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