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  • Title: [Effect of T3 on the expression of transferrin receptor and ferritin in K562 cells and its possible mechanism].
    Author: Zhou M, Liao QK, Li FY, Li Q, Luo CH, Gao J, Jia CS, Yang CL.
    Journal: Zhonghua Xue Ye Xue Za Zhi; 2003 Apr; 24(4):181-4. PubMed ID: 12864947.
    Abstract:
    OBJECTIVE: To explore the effect of T(3) on the expression of transferrin receptor (TfR) and ferritin (Fn) in K562 cells and its possible mechanism. METHODS: Flow cytometry was used for the detection of TfR expression, radioimmunoassay for Fn expression, RNA/protein band shift assay for the binding activity of iron regulatory protein (IRP) and iron responsive elements (IRE), and RT-PCR for TfR and Fn mRNA levels. RESULTS: Different concentration of T(3) significantly increased Fn expression of K562 cells, especially at 100 nmol/L and 200 nmol/L (p < 0.05). However, T(3) had no effect on TfR expression. T(3) decreased the binding activity between IRP and IRE, particularly at concentration of 50 nmol/L. Different concentration of T(3) increased Fn-H mRNA level at different time point while it had no effect on TfR mRNA level. CONCLUSION: T(3) increased Fn expression of K562 cells through the possible mechanisms of either the post-transcriptional regulation or transcriptional modulation.
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