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  • Title: Evaluation of a commercially available ELISA kit as a tool to determine BTEX in groundwater.
    Author: Francioni E, Fillmann G, Hamacher C, Wagener Ade L, Depledge MH, Readman JW, Meniconi Mde F.
    Journal: Environ Technol; 2003 Jun; 24(6):665-70. PubMed ID: 12868520.
    Abstract:
    The reliability of enzyme-linked immunosorbent assay (ELISA) tests as a screening technique to address groundwater contamination was tested in an area following leakage of gasoline from a petrol station. Immunoassay data of benzene, toluene, ethylbenzene, and o-, m- and p-xylene (BTEX) were compared with results obtained using capillary gas chromatographic analysis. Detection limits were of 20 microg l(-1) for ELISA and 0.3 microg l(-1) for gas chromatography with flame ionization and photoionization detectors (GC-FID/PID) determination. Despite an observed overestimation of BTEX concentrations as given by ELISA, the tests responded reliably to different levels of contamination.
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