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  • Title: Quantitative determination of sugars and myo-inositol in citrus fruits grown in Japan using high-performance anion-exchange chromatography.
    Author: Masuda T, Kawano A, Kitahara K, Nagashima K, Aikawa Y, Arai S.
    Journal: J Nutr Sci Vitaminol (Tokyo); 2003 Feb; 49(1):64-8. PubMed ID: 12882398.
    Abstract:
    Fifteen commercial samples of citrus fruits grown in Japan were analyzed for their sugar contents by high-performance anion-exchange chromatography (HPAEC) and electrochemical detection (ED) coupled with a stationary phase D10 column prepared using chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyldiaminodecane. Myo-inositol, glucose, fructose, and sucrose in all of these various citrus fruits could be successfully separated within 25 min using 0.5 M NaOH eluent at a flow-rate of 0.4 mL/min. Myo-inositol, as a better nutritional source, was found in all the citrus fruits grown in Japan (0.7 +/- 0.04-2.1 +/- 0.2 g/L). The sugar contents of twelve citrus fruits grown in other countries were also determined.
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