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  • Title: [Identification of Taenia saginata by mtCO I in four areas of Yunnan and Guizhou provinces].
    Author: Wang ZR, Bao HE.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 2003; 21(1):20-3. PubMed ID: 12884584.
    Abstract:
    OBJECTIVE: To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province. METHODS: Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunit I (mtCO I) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package. RESULTS: The mtCO I gene sequences of Lanping sample were identical to that of T. saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCO I gene sequences, while the sample from Congjiang had the same mtCO I gene sequences as T. saginata. The homology between these two groups of gene sequences was 97.44%, while the homology of amino acid sequences reached to 99.16%. The constructed phylogenetic tree revealed that the relationship between T. saginata asiatica and T. saginata is closer, both are distant relative to T. solium and other species of cestodes. CONCLUSION: The Taenia prevalent in Lanping, Dali and Duyun is identified as T. saginata asiatica, while that isolated in Congjiang is the typical T. saginata.
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