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  • Title: [Microfluorimetric research on the erythroblastic islands and macrophages of the bone marrow].
    Author: Rassokhin AG, Pochinskiĭ AG, Liannoĭ KV, Ovchinnikov GA.
    Journal: Biull Eksp Biol Med; 1992 Oct; 114(10):347-51. PubMed ID: 1288682.
    Abstract:
    A microfluorimetric system was used to study rat bone marrow erythroblastic islands (EI) and macrophages. Measurement of fluorescence from acridine-orange-treated cells was performed on the microscope LUMAM-E3 (LOMO, Leningrad) at 530-550 and 630-650 nm. The intensity of fluorescence in 530-550 nm range depended on the intensity of proliferative processes in EI, that at 630-650 nm was associated with activity of lysosomal apparatus of EI macrophages. Erythroblast amplification in EI is parallel both to enhancement of fluorescence intensity at 530-550 nm and 630-650 nm. Intensity of fluorescence of different bone marrow macrophages was evaluated. It is suggested that a part of bone marrow macrophages has high affinity to erythroid tissue.
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