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Title: Functional identification of rub52 gene involved in the biosynthesis of rubradirin. Author: Maharjan J, Liou K, Lee HC, Kim CG, Lee JJ, Yoo JC, Sohng JK. Journal: Biotechnol Lett; 2003 Jun; 25(11):909-15. PubMed ID: 12889805. Abstract: An open reading frame, rub52, has been identified as a gene encoding thymidine diphospho-glucose 2,3-dehydratase by sequence analysis of the rubradirin biosynthetic gene cluster of Streptomyces achromogenes var. nibradiris NRRL3061. The gene codes for a protein consisting of 458 amino acids with calculated molecular mass of 50862 Da. The gene was amplified and heterologously expressed in Escherichia coli as a soluble His-tagged fusion protein. C-2 deoxygenation functionality of thymidine diphospho-4-keto-6-deoxyglucose was assigned to the rub52 gene product from in vitro enzyme assay.[Abstract] [Full Text] [Related] [New Search]