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  • Title: [The effect of telomerase inhibitors on oral squamous carcinoma cells].
    Author: Yang Z, Chen W, Zhang Q.
    Journal: Hua Xi Kou Qiang Yi Xue Za Zhi; 2003 Jun; 21(3):231-4. PubMed ID: 12898773.
    Abstract:
    OBJECTIVE: To study the potential anti-tumor effect of telomerase inhibitors. METHODS: Human oral squamous carcinoma cell line KB was selected as target cell. The effects of 3'-azido-3'-deoxythymidine (AZT) and human antisense phosphorothioate (AS-ONS) for telomerase template on KB cell line were investigated. The cytotoxic effect of AZT and AS-ONS on tumor cells was quantified using MTT colorimetric assay. Assay of 3H-TdR incorporation was undertaken to measure the cell proliferation. The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi-quantitative analysis. The Flow Cytometry was used to detect apoptosis and measure cell cycle. RESULTS: Both AZT and AS-ONS inhibited the growth of KB cell line in a certain range of concentrations, and meanwhile the telomerase activity was reduced after treatment. In addition, both AZT and AS-ONS can induce apoptosis and arrest G1 phase of cell cycle. CONCLUSION: The results obtained above indicated that AZT and AS-ONS could be potentially used as an anti-oral carcinoma agent or an auxiliary treatment for cancer. Those inhibitory effects might be partially due to the induction of apoptosis and the prolongation of cell cycle.
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