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  • Title: The role of nucleosome positioning in repression by the yeast alpha 2/Mcm1p repressor.
    Author: Saito S, Miura S, Yamamoto Y, Shindo H, Shimizu M.
    Journal: Nucleic Acids Res Suppl; 2002; (2):93-4. PubMed ID: 12903121.
    Abstract:
    In Saccharomyces cerevisiae, alpha 2/Mcm1p represses a-cell specific genes. Previous studies indicated that repression by alpha 2/Mcm1p is associated with organization of positioned nucleosomes in promoters of a-cell specific genes such as STE6 and BAR1. Here, we examined the role of nucleosome positioning in repression of STE6-lacZ by alpha 2/Mcm1p. We showed that insertion of an A34 sequence, which is shown to disrupt an array of positioned nucleosomes, affects STE6-lacZ expression. Furthermore, artificial recruitment of TBP is facilitated by the insertion of A34 to derepress STE6-lacZ expression. These findings support the model that nucleosome positioning plays a regulatory role in repression by alpha 2/Mcm1p.
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