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  • Title: [Gene clone and its characteristics on band 7-like protein in Plasmodium falciparum FCC1/HN].
    Author: Zhang L, Zhang LH, Wang HY, Wang H.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 2003 Apr; 25(2):181-4. PubMed ID: 12905716.
    Abstract:
    OBJECTIVE: To identify and clone the gene named pfstom gene which encoding the protein belonging to band 7 family and to do primary research on its function. METHODS: Based on the finished data in international public malaria database, coding sequence of pfstom cDNA was obtained by RT-PCR from FCC1/HN. Its phylogenetic profiles and the homogeny were analyzed by some softwares. After Prokaryotic expression, C terminal of Pfstom protein was expressed by Pet30a system. Recombinant Pfstom protein was used to immol/Lunize rabbit and then serum was harvested and the IgG was purified for Western blot. RESULTS: The coding sequence of pfstom is 1,125 bp which encoding 374 amino acids with C-terminal fragment being homogenous to stomatin-like protein which belongs to band 7 family. Phylogenetic profiles analysis revealed its homogeny to stomatin. Western blot showed its stage-specific expression in trophozoite. CONCLUSION: Pfstom belongs to band-7 family. It was expressed specifically in trophozoite in erythrocyte stage of plasmodium falciparum. It was not expressed in ring stage. And it is membrane-related protein. All these results provided the foundation for further research on pfstom.
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