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  • Title: Arginine selective biosensor based on arginase-urease immobilized in gelatin.
    Author: Karacaoğlu S, Timur S, Telefoncu A.
    Journal: Artif Cells Blood Substit Immobil Biotechnol; 2003 Aug; 31(3):357-63. PubMed ID: 12906315.
    Abstract:
    Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross-linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris-HCl buffer (pH 8.5) was used at 25 degrees C. The biosensor response depends linearly on arginine concentration between 0.025-0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.
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