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  • Title: [The reactivity of the circulating neutrophils in human peripheral blood].
    Author: Bakuev MM, Zurkhaeva RZ.
    Journal: Biull Eksp Biol Med; 1992 Nov; 114(11):542-4. PubMed ID: 1290838.
    Abstract:
    Using cytochemical, biochemical and disc-electrophoretic methods, the degree of extracellular secretion of peroxidase-containing neutrophil granules has been investigated as an index of their functional activity when in contact with antigens of extra- and intra-circulation. It was established that in in vitro contacts of neutrophils with alive and killed microbe culture St. aur., the quantity of the granules in the cells decreased as well as the enzyme activity in them. This is partially due to extracellular secretion of the granules content confirmed by the presence of peroxidase fractions in the solution. Similar results have been obtained for circulating neutrophils and serum of patients with acute pneumonia at the height of the disease. It is hold that antigen-induced extra- and intracellular neutrophil degranulation in the peripheral human blood reflects functional activity of the neutrophils.
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