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  • Title: Expression and characterization of human interferon-beta1 in the methylotrophic yeast Pichia pastoris.
    Author: Skoko N, Argamante B, Grujicić NK, Tisminetzky SG, Glisin V, Ljubijankić G.
    Journal: Biotechnol Appl Biochem; 2003 Dec; 38(Pt 3):257-65. PubMed ID: 12911337.
    Abstract:
    We describe the heterologous expression of a human interferon-beta1 in the methylotrophic yeast Pichia pastoris. Biologically active recombinant human interferon-beta1 (rHuIFN-beta1) was secreted from shake-flask-grown P. pastoris cells into the medium using the Saccharomyces cerevisiae alpha-mating factor prepro-leader sequence at the level of (1-3) x 10(5) i.u. (international units)/ml (6-12 mg/litre). An rHuIFN-beta1 with an N-terminal sequence identical with that of native HuIFN-beta1 was purified and the specific activity was determined (2-3 x 10(7) i.u./mg). It was found that the secreted recombinant protein was partially N-glycosylated.
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