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  • Title: Changes in salivary cortisol concentrations during a 24-hour period in dogs.
    Author: Koyama T, Omata Y, Saito A.
    Journal: Horm Metab Res; 2003 Jun; 35(6):355-7. PubMed ID: 12920657.
    Abstract:
    The purpose of this investigation was to clarify whether salivary cortisol secretion in dogs had a circadian rhythm. Saliva sampling during a 24-hour period was performed in 4 non-consecutive days. Eight adult beagle dogs (4 males and 4 females) were divided into 4 groups, and 2 dogs (1 male and 1 female) were used for each repetition. Saliva samples were taken at 1 h intervals from 9:00 a. m. to 9:00 a. m. of the following day. Salivary cortisol concentrations were determined using enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary cortisol, and the differences among salivary cortisol concentrations measured every hour were not demonstrated during a 24-hour period in dogs.
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