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  • Title: A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase.
    Author: Geiger R, Schneider E, Wallenfels K, Miska W.
    Journal: Biol Chem Hoppe Seyler; 1992 Dec; 373(12):1187-91. PubMed ID: 1292503.
    Abstract:
    A derivative of D-luciferin, D-luciferin-O-beta-galactoside, was synthesized and used as highly sensitive substrate for beta-galactosidase. The substrate was physicochemically characterized. Enzymatic cleavage of the new compound by beta-galactosidase was demonstrated and kinetic constants Km, Vmax, kcat and kcat/Km have been determined. The compound has been proved to be a highly sensitive substrate for beta-galactosidase, permitting a limit of detection of 3.7 x 10(-19) mol of enzyme per assay.
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