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  • Title: Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.
    Author: Shimamura T, Koike-Takeshita A, Yokoyama K, Yoshida M, Taguchi H, Iwata S.
    Journal: Acta Crystallogr D Biol Crystallogr; 2003 Sep; 59(Pt 9):1632-4. PubMed ID: 12925795.
    Abstract:
    The chaperonin GroEL-GroES (GroEL/ES) complex from a thermophilic eubacteria, Thermus thermophilus HB8, has been purified and crystallized. The GroEL/ES complex is known to be composed of 14 identical GroEL subunits (58 kDa) and seven identical GroES subunits (11 kDa). The GroEL/ES complex crystals belong to the triclinic space group P1, with unit-cell parameters a = 140.4, b = 156.4, c = 273.1 A, alpha = 82.9, beta = 85.4, gamma = 68.5 degrees. The crystal asymmetric unit contains two molecules (MW = 885 kDa). One data set to 3.0 A resolution, with 383 652 independent observations (89.3% complete) and an R(merge) of 0.08, has been collected from a single crystal. A molecular-replacement solution was obtained using the structure of the GroEL/ES complex from Escherichia coli as a search model.
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