These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo.
    Author: Saunders A, Werner J, Andrulis ED, Nakayama T, Hirose S, Reinberg D, Lis JT.
    Journal: Science; 2003 Aug 22; 301(5636):1094-6. PubMed ID: 12934007.
    Abstract:
    RNA polymerase II (Pol II) transcription through nucleosomes is facilitated in vitro by the protein complex FACT (Facilitates Chromatin Transcription). Here we show that FACT is associated with actively transcribed Pol II genes on Drosophila polytene chromosomes. FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6. Interestingly, FACT does not colocalize with Pol III-transcribed genes, which are known to undergo nucleosome transfer rather than disassembly in vitro. Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms.
    [Abstract] [Full Text] [Related] [New Search]