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  • Title: [Diagnosis of several common alpha-thalassemia genotypes by multiplex fluorescence polymerase chain reaction].
    Author: Wen S, Wu LQ, Li Q.
    Journal: Hunan Yi Ke Da Xue Xue Bao; 2003 Apr; 28(2):123-6. PubMed ID: 12934354.
    Abstract:
    OBJECTIVE: To develop an approach to a quick and accurate genetic diagnosis. METHODS: Byusing fluorescence primer, the fragments of alpha 1 and alpha 2-globin gene and internal control: beta-actin were amplified and then the PCR products were analyzed with Perkin Elmer ABI PRISMTM 377DNA Sequencer. Linkage analysis was also performed with (CA)n at the upstream of alpha 2-globin gene. The two methods were compared. RESULT: The fluorescence polymerase chain reaction and the linkage analysis gave the same result, and it was easy to distinguish different genotypes. CONCLUSION: The multipilx fluorescence polymerase chain reaction is simple, sensitive and feasible for rapid diagnosis of alpha thalassemia, and may be utilized for the genetic diagnosis of the pre-transplantation and the diagnosis by fetal nucleated erythrocytes in maternal blood.
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