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  • Title: Gene expression and tyrosine kinase activity of insulin receptor in uterine leiomyoma and matched myometrium.
    Author: Toscani GK, Chaves EM, Cervi FL, Tavares MB, Silva IS, von Eye Corleta H, Capp E.
    Journal: Arch Gynecol Obstet; 2004 Nov; 270(3):170-3. PubMed ID: 12937923.
    Abstract:
    OBJECTIVE: Our objective was to determine the insulin receptor (IR) mRNA levels and IR tyrosine kinase activity in normal myometrium and leiomyoma. DESIGN: Experimental study. SETTING: Academic research center. PATIENTS: Five women with leiomyoma submitted to hysterectomy. INTERVENTION: Plasma membrane fraction of human myometrium and leiomyoma were prepared and samples were incubated with and without insulin. mRNA was isolated and RT-PCR with specific primers was performed. MAIN OUTCOME MEASURE: Western blots of plasma membranes incubated with and without insulin were performed. Chemoluminescent methods followed by densitometry were used to assess IR autophosphorylation. RT-PCR with specific primers for IR gene sequences was used to determine IR mRNA levels. RESULTS: IR mRNA levels in myometrium (0.634+/-0.038) and in leiomyoma (0.649+/-0.047; p=0.813) were not different. The degree of insulin-stimulated IR autophosphorylation (relative optical density of the 95 kDa band) was also not different between myometrium (1.496+/-0.310) and leiomyoma (1.593+/-0.129; p=0.650). CONCLUSIONS: There was no difference in IR receptor expression and IR autophosphorylation between normal myometrium and leiomyoma. Other steps of insulin signaling chain may participate in the altered proliferation of leiomyomas.
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