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  • Title: [Chromatographic behavior of fat-soluble vitamin in reversed phase liquid chromatography with water-isopropanol mobile phase].
    Author: Ha SE, Ta YE.
    Journal: Guang Pu Xue Yu Guang Pu Fen Xi; 2002 Aug; 22(4):693-4. PubMed ID: 12938404.
    Abstract:
    The separation of a mixture fat-soluble vitamin A, D, E by reversed-phase HPLC on elution with an isopropanol-water was studied. A procedure was developed for determining vitamin mixtures and was applied to the analysis of 9 multivitamin tablet. The vitamin was fully separated in 15 min using a short C18 column and UV detection at 280 nm. Different solvent were used for sample extraction. The recoveries were measured, and were higher than 95%. Experimental results proved that the three fat-soluble vitamin A, D, E all can be fully eluted by a solution of isopropanol-water in the ratio of 75%:25%.
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