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  • Title: Comparison of cryopreparation techniques for electron probe microanalysis of cells as exemplified by human erythrocytes.
    Author: Zierold K.
    Journal: Scanning Microsc; 1992 Dec; 6(4):1137-43; discussion 1143-5. PubMed ID: 1295083.
    Abstract:
    Erythrocytes in human blood were used to evaluate the reliability of cryopreparation techniques for electron probe X-ray microanalysis of biological cells and tissues. The elemental content determined by X-ray microanalysis of ultrathin freeze-dried cryosections was found to be consistent with data known from the literature. Considerable redistribution of the intracellular elemental composition was found after freeze-substitution as well as after freeze-drying followed by resin embedding. Two conclusions are drawn from this study: 1. Erythrocytes in human blood are a suitable reference specimen for evaluation of specimen preparation techniques for microanalysis. 2. At present, freeze-dried cryosections are the most reliable specimen type for quantitative electron probe microanalysis of cells.
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