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  • Title: Measurement of molt-inhibiting hormone titer in hemolymph of the American crayfish, Procambarus clarkii, by time-resolved fluoroimmunoassay.
    Author: Nakatsuji T, Sonobe H.
    Journal: Zoolog Sci; 2003 Aug; 20(8):999-1001. PubMed ID: 12951406.
    Abstract:
    In order to determine the titer of molt-inhibiting hormone (Prc-MIH) in the hemolymph of the American crayfish Procambarus clarkii, a time-resolved fluoroimmunoassay (TR-FIA) was established using specific antibodies against N-terminal and C-terminal segments of Prc-MIH. The lowest limit of detection of Prc-MIH in TR-FIA was 10 amol/assay. The Prc-MIH titers in the hemolymph were 6.53 fmol/ml at the intermolt stage and 1.25 fmol/ml at the early premolt stage. This result is consistent with the long-known hypothesis that the Y-organ is inhibited by MIH during the intermolt stage, whereas the Y-organ is activated by being freed from the inhibitory regulation of MIH.
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