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  • Title: [Transformation of wml1 5' promoter region into tomato plants and studies on its transcriptional regulation role].
    Author: Wu HY, Liu JM, Zhu ZJ, Yang XT, Chen DM.
    Journal: Shi Yan Sheng Wu Xue Bao; 2003 Jun; 36(3):226-32. PubMed ID: 12966734.
    Abstract:
    According to its restriction sites, fragments of 1573 bp, 1197 bp, 896 bp and 795 bp were obtained from the 5' promoter region of wml1 and fused with the coding sequence of the GUS gene. Constructs containing these fragments were introduced into tomato plants via Agrobacterium-mediated transformation. Histochemical assay of GUS expression in transgenic tomato plants revealed that fragments of 1573 bp, 1197 bp, 896 bp were able to direct GUS expression in fruits of 15, 30, 45 days after anthesis with the expression level of GUS increasing with fruit development, but not in leaves, stems and roots. While no GUS expression was observed in tomato plants transformed by construct containing fragment of 795 bp. It was determined that the region from 857 bp to 957 bp contains the elements necessary for directing fruit-specific expression.
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