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  • Title: An indirect fluorescent antibody (IFA) test for the serodiagnosis of Kala-Azar.
    Author: Choudhry A, Puri A, Guru PY, Saxena RP, Saxena KC.
    Journal: J Commun Dis; 1992 Mar; 24(1):32-6. PubMed ID: 1296950.
    Abstract:
    The IFA test developed using Leishmania donovani promastigote and amastigote antigens showed very high antibody titre in clinically and parasitologically established cases (30) of kala-azar, the geometrical mean reciprocal titre (GMRT) being 870 +/- 5.4 and 5370 +/- 1.80 respectively with the two antigens. In contrast, the GMRT of normal subjects of endemic area was only 12.44 +/- 6.19 and 80.35 +/- 1.66 respectively with these antigens. None of the subjects from non-endemic area suffering with other parasitic diseases, such as malaria, filaria, amoebiasis, leprosy or tuberculosis (20 cases each) gave a positive response to any of the antigen. The test holds promise in the diagnosis of Kala-azar.
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