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  • Title: [Problems encountered in identification of penicillin binding proteins with pneumococcus used as an example].
    Author: Markiewicz Z.
    Journal: Med Dosw Mikrobiol; 1992; 44(1-2):5-11. PubMed ID: 1297035.
    Abstract:
    Whole cells or isolated membranes of Streptococcus pneumoniae were treated with labelled benzyl penicillin and the penicillin binding proteins (PBPs) were visualized by fluorography after SDS-PAGE electrophoresis. The PBP profiles obtained for strains sensitive and resistant to penicillin strongly differed depending not only on the concentrations of acrylamide and bis-acrylamide used in the separating gel but also on the batch used (different manufacturers). The latter was also true for sodium dodecyl sulfate.
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