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  • Title: [Preservation of Pseudomonas putida bacteria at low temperatures].
    Author: Vysekantsev IP, Krasheninnikova TK, Olekhnovich EV, Stepaniuk LV.
    Journal: Mikrobiologiia; 1992; 61(6):1098-100. PubMed ID: 1297044.
    Abstract:
    We have found that the mode of cooling, composition of cryopreservation medium, original concentration of cells and storage temperature affect viability of Pseudomonas putida bacteria during low-temperature preservation. We have elaborated the conditions of preservation, providing for a high survival of bacteria, namely: one-stage cooling with the rates of 30, 40 degrees C/min or immersion into liquid nitrogen in the culturing medium with addition of sucrose, glycerol or dimexide in the concentration of 0.5 M; storage temperature is -80 degrees C divided by -196 degrees C.
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