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  • Title: [Selective apoptosis in HeLa cells induced by sodium butyrate and its mechanism].
    Author: Xi L, Wu MF, Feng YD, Wang W, Lu YP, Zhou JF, Ma D.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 2003 Aug; 25(4):401-5. PubMed ID: 12974082.
    Abstract:
    OBJECTIVE: To investigate apoptosis induced by sodium butyrate in cervix cancer cell line HeLa and primary human embryo lung fibroblasts and its mechanism. METHODS: Cell apoptosis was assessed by morphology, cell viability, DNA fragmentation, the percentage of sub-G1 cells and phosphatidylserine (PS) externalization. The effects of sodium butyrate on transcription of Bax and Bcl-2 was analyzed by RT-PCR. RESULTS: Sodium butyrate inhibited proliferation in a time and dose-dependant manner. The inhibition of proliferation in HeLa cells was more significant than that in primary human embryo lung fibroblasts. DNA fragmentation, sub-G1 peak and AnnexinV/PI by flow cytometry showed very high apoptosis rates in HeLa cells 72 hours after treated with sodium butyrate, while pretty low in primary human embryo lung fibroblasts. RT-PCR showed sodium butyrate had little effects on transcription of Bax and Bcl-2 in HeLa cells. CONCLUSION: Sodium butyrate can induce apoptosis in HeLa cells without changing the expression of Bax and Bcl-2. Sodium butyrate comparatively has little effects on fibroblasts.
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