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  • Title: Specific insulin binding by, and imprintability of, the nuclear membrane of Tetrahymena.
    Author: Hegyesi H, Csaba G.
    Journal: Cytobios; 1992; 72(290-291):153-7. PubMed ID: 1298579.
    Abstract:
    Cellular nuclei isolated from Tetrahymena were able to bind FITC-labelled insulin, and pretreatment with different concentrations of unlabelled insulin accounted for a decrease in FITC-insulin binding of the nuclear membrane. This strongly suggests an association of specific insulin receptors with the nuclear envelope of Tetrahymena. Nuclei isolated 1 week after primary exposure of Tetrahymena cells to insulin showed a 50% binding increase over the controls. This supports the implication that hormonal (insulin) imprinting also involves the receptors of the nuclear membrane of the imprinted cells.
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