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  • Title: Changes in proteolytic susceptibility of human erythrocyte membrane proteins during red blood cell aging.
    Author: Gaczyńska M.
    Journal: Cytobios; 1992; 72(290-291):197-200. PubMed ID: 1298582.
    Abstract:
    The method employed facilitated analysis of the digestion of denatured proteins, depleted in their natural membrane environment. The susceptibility of human erythrocyte membrane proteins to digestion with trypsin was studied by the two-dimensional electrophoretic method. Ghosts were isolated from erythrocytes fractionated according to density (age). Spectrin and band 3 protein from membranes isolated from the youngest cells were significantly more slowly degraded than proteins from middle-age cell membranes and from the oldest cells.
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