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  • Title: [A method of isolating inhibitors of the proliferative activity of liver hepatocytes].
    Author: Okulov VB, Chekulaeva LI.
    Journal: Arkh Anat Gistol Embriol; 1976 Jan; 70(1):106-9. PubMed ID: 130098.
    Abstract:
    A fraction containing two tissue specific regulators of the mitotic activity (chalone) and having a definite capacity to inhibit the DNA synthesis (G1-inhibitor) and to enter into the mitosis (G2-inhibitor) of hepatocytes of the regenerating liver was isolated from an aqueous extract by ethanol saturation from 70 to 81%. The fraction contains up to 10 substances of protein nature, 7 of which, due to the data of immunological analysis, are also present in other tissues and blood serum of rats.
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