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  • Title: [The isolation of nucleases Sm1 and Sm2 in preparative amounts and a study of their antigenicity].
    Author: Filimonova MN, Urazov NG, Khaertynov KS.
    Journal: Nauchnye Doki Vyss Shkoly Biol Nauki; 1992; (2):65-70. PubMed ID: 1302504.
    Abstract:
    Two enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.
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