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  • Title: Blood grouping of minute samples using monoclonal anti-A,B antibody.
    Author: Aoki Y, Nagae M, Nata M, Sagisaka K.
    Journal: Nihon Hoigaku Zasshi; 1992 Dec; 46(6):436-9. PubMed ID: 1303449.
    Abstract:
    The availability of a monoclonal anti-A,B antibody (AB-E6E2) for blood grouping of blood and saliva stains was investigated. The antibody, which was reacted with A, B and AB antigens but not with O antigen, was produced from BALB/c mouse-mouse hybridoma raised to group AB red blood cells. AB-E6E2 had heat-elutability enough to detect ABO antigens in blood and saliva stains by absorption-elution test, whereas it had extremely weak double combining potency in mixed agglutination test. Application of elution-ELISA method will enable the detection of smaller amount of ABH antigens. The antibody, therefore, would be useful to segregate group O minute samples from the other blood group ones.
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