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  • Title: A directional, high-frequency chromosomal mobilization system for genetic mapping of Rhizobium meliloti.
    Author: Klein S, Lohman K, Clover R, Walker GC, Signer ER.
    Journal: J Bacteriol; 1992 Jan; 174(1):324-6. PubMed ID: 1309521.
    Abstract:
    A system for mapping of the Rhizobium meliloti chromosome that utilizes transposon Tn5-Mob, which carries the mobilization site of IncP plasmid RP4 (R. Simon, Mol. Gen. Genet. 196:413-420, 1984), was developed. Insertions of Tn5-Mob that were located at particular sites on the R. meliloti chromosome were isolated and served as origins of high-frequency chromosomal transfer when IncP tra functions were provided in trans. This approach is, in principle, applicable to any gram-negative bacterium in which Tn5 can transpose and into which IncP plasmids can conjugate.
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