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  • Title: Regulation of interleukin-1 receptors on AtT-20 mouse pituitary tumour cells.
    Author: Kobayashi H, Fukata J, Tominaga T, Murakami N, Fukushima M, Ebisui O, Segawa H, Nakai Y, Imura H.
    Journal: FEBS Lett; 1992 Feb 17; 298(1):100-4. PubMed ID: 1312014.
    Abstract:
    To study the cellular mechanisms of interleukin-1 (IL-1) in the pituitary corticotroph, we studied the properties of IL-1 receptors on a mouse pituitary ACTH-producing cell line, AtT-20. Scatchard plot analysis revealed a single type of receptor with a Kd (dissociation constant) of 93 pM, and 482 binding sites/cell. [125I]IL-1 alpha binding competed with IL-1 alpha and IL-1 beta in an equimolar fashion. A 24 h pre-incubation with either CRH, epinephrine or nor-epinephrine increased the [125I]IL-1 alpha binding sites in the AtT-20 cells and conversely, a similar pre-incubation with either dexamethasone or tumour necrosis factor-alpha (TNF alpha) decreased them without affecting the affinity of the receptors in either case.
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