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  • Title: Autocrine receptors for endothelins in the primary culture of endothelial cells of human umbilical vein.
    Author: Fujitani Y, Oda K, Takimoto M, Inui T, Okada T, Urade Y.
    Journal: FEBS Lett; 1992 Feb 17; 298(1):79-83. PubMed ID: 1312017.
    Abstract:
    Human umbilical vein endothelial cells (HUVECs) in primary culture produced and secreted endothelin 1 (ET-1) actively. Specific binding of [125I]ET-1 to these cells was not detectable because of the saturation of ET receptors with endogenously produced ET-1. However, addition of phosphoramidon, an inhibitor of ET-converting enzyme, to the medium reduced the production of ET-1 and thus the receptors on HUVECs were made available for exogenously added [125I]ET-1. Binding studies using phosphoramidon-treated HUVECs indicated the existence of a non-isopeptide-selective type (ETB) of ET receptor with a Kd of 17 pM. This receptor is thought to be involved in ET-induced vasodilation in an autocrine manner in vivo.
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