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  • Title: Detection of elsamicin-DNA binding specificity by restriction enzyme cleavage.
    Author: Párraga A, Portugal J.
    Journal: FEBS Lett; 1992 Mar 23; 300(1):25-9. PubMed ID: 1312486.
    Abstract:
    The sequence specificity of elsamicin A, an anti-tumour antibiotic, binding to DNA was elucidated considering the inhibition of the rate of digestion of linearised pBR322 DNA by AatII, ClaI, EcoRI, HindIII and NruI restriction enzymes. Elsamicin A inhibits the rate of digestion by NruI (recognition sequence TCG/CGA) to a greater extent than it does for the other enzymes, thus evidencing the sequence-selective binding of elsamicin to CGC regions in DNA. Our results also show the important role of the neighbouring sequences in the elsamicin A-DNA interactions and their effects on the cleavage by restriction enzymes.
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