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  • Title: Detection of antibodies to ovine lentivirus using a recombinant antigen derived from the env gene.
    Author: Kwang J, Cutlip R.
    Journal: Biochem Biophys Res Commun; 1992 Mar 31; 183(3):1040-6. PubMed ID: 1314572.
    Abstract:
    The Western blot assay was performed to characterize antibodies to the transmembrane glycoprotein (TGP) of ovine progressive pneumonia virus (OPPV) by using glutathione-S-transferase-TGP (GST-TGP) protein. The GST-TGP protein was efficiently expressed in Escherichia coli (E. coli) and was highly immunoreactive in the Western blot assay. This assay detected antibodies in 97% (103/106) of the sera from agarose gel immunodiffusion (AGID) positive OPP animals. Like human AIDS patients, antibodies to TGP appear to be one of the major serological markers in OPP infected animals.
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