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  • Title: Infection of Chang cells with hepatitis C virus using hepatic biopsy specimens from patients with chronic hepatitis (type C).
    Author: Ozeki T, Hikiji K, Funakoshi K, Tsutsumi M, Yoshida A.
    Journal: Int J Exp Pathol; 1992 Feb; 73(1):1-8. PubMed ID: 1315565.
    Abstract:
    The presence of hepatitis C virus sequence was detected in liver tissue extracts by the polymerase chain reaction (PCR) method using primers of non-coding region in six out of eight cases with chronic hepatitis seropositive for Chiron's antibody. Subsequently, liver extracts from these cases were added to cell cultures of Chang cells for 3 days. The liver extracts of the six cases positive for PCR appeared to infect the Chang cells.
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