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  • Title: Expression of the gene coding for the major outer capsid protein of SA-11 rotavirus in a baculovirus system.
    Author: McGonigal TP, Turon MC, Komar-Hartnett K, Kister SE, Smith RE.
    Journal: Virus Res; 1992 Apr; 23(1-2):135-50. PubMed ID: 1318623.
    Abstract:
    The gene coding for the major outer capsid protein (VP7) of simian rotavirus SA-11 has been expressed in a baculovirus-insect cell system. The resulting protein is 35 kDa and is primarily associated with the endoplasmic reticulum. Neutralizing SA-11 polyclonal antiserum and VP7 monospecific antiserum reacted specifically with the expressed gene product. Antiserum derived against the recombinant VP7 protein neutralized SA-11 rotavirus infectivity in a fluorescent focus assay.
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