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  • Title: Cloning and expression of a member of the Aspergillus niger gene family encoding alpha-galactosidase.
    Author: den Herder IF, Rosell AM, van Zuilen CM, Punt PJ, van den Hondel CA.
    Journal: Mol Gen Genet; 1992 Jun; 233(3):404-10. PubMed ID: 1320186.
    Abstract:
    An enzyme with alpha-galactosidase activity and an apparent molecular weight of 82 kDa was purified from culture medium of Aspergillus niger. The N-terminal amino acid sequence of the purified protein shows similarity to the N-terminal amino acid sequence of alpha-galactosidases from several other organisms. Oligonucleotides, based on the N-terminal amino acid sequence, were used as probes to clone the corresponding gene from a lambda EMBL3 gene library of A. niger. The cloned gene (aglA) was shown to be functional by demonstrating that the 82 kDa alpha-galactosidase is absent from a strain with a disruption of the aglA gene, and is over-produced in strains containing multiple copies of the aglA gene. Enzyme activity assays revealed that the 82 kDa alpha-galactosidase A represents a minor extracellular alpha-galactosidase activity in A. niger.
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