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  • Title: Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies.
    Author: Tozzini F, Matteucci D, Bandecchi P, Baldinotti F, Poli A, Pistello M, Siebelink KH, Ceccherini-Nelli L, Bendinelli M.
    Journal: J Virol Methods; 1992 Jun; 37(3):241-52. PubMed ID: 1321834.
    Abstract:
    The feline immunodeficiency virus (FIV) readily produced syncytia in Crandell feline kidney (CrFK) cells adapted to a medium containing 0.5% fetal calf serum, a variety of growth factors and other supplements. This finding has been exploited to develop simple and sensitive virus titration and neutralization assays. High titre neutralizing antibodies were detected in cats infected naturally and experimentally with FIV, but not in uninfected animals.
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