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  • Title: Platinum-diaminobenzidine reaction and its contribution to the quantitation of cytochrome oxidase activity.
    Author: Hiraoka T, Hirai K.
    Journal: J Electron Microsc (Tokyo); 1992 Apr; 41(2):127-9. PubMed ID: 1324291.
    Abstract:
    The platinum-diaminobenzidine (Pt-DAB) reaction, which yields a black, electron-dense, insoluble, Pt-containing reaction product at the active site of cytochrome oxidase without postosmification, has been developed. This reaction permits us to quantitate the oxidase activity in an individual mitochondrion or in its constitutent part by the energy dispersive X-ray analysis (EDAX), based on the platinum atoms incorporated in the product.
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