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  • Title: Purification and reconstitution of mu-opioid receptors in liposome.
    Author: Fujioka T, Inoue F, Sumita S, Kuriyama M, Hanawa T, Katagi T.
    Journal: J Chromatogr; 1992 Apr 24; 597(1-2):429-33. PubMed ID: 1325467.
    Abstract:
    Opioid receptors solubilized from rat brain membranes with digitonin were partially purified with a newly prepared affinity resin, AF-Amino Toyopearl, coupled with a mu-antagonist Tyr-Pro-Tyr-Tyr at the C-terminus of the peptide. The purified materials were reconstituted with an inhibitory GTP-binding protein (Gi) in liposome. From displacement analyses, two binding states, with a high and a low affinities for the mu-agonist [D-Ala2,Me-Phe4,Gly-ol5]enkephalin, were observed in the reconstituted system with Gi, only a low-affinity state was observed in the reconstituted system without Gi. The results suggested that the purified materials contained the mu-opioid receptors and could functionally couple with Gi as observed in the cell membranes.
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