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Title: Saturation kinetics of coenzyme Q in NADH and succinate oxidation in beef heart mitochondria. Author: Estornell E, Fato R, Castelluccio C, Cavazzoni M, Parenti Castelli G, Lenaz G. Journal: FEBS Lett; 1992 Oct 19; 311(2):107-9. PubMed ID: 1327877. Abstract: The saturation kinetics of NADH and succinate oxidation for Coenzyme Q (CoQ) has been re-investigated in pentane-extracted lyophilized beef heart mitochondria reconstituted with exogenous CoQ10. The apparent 'Km' for CoQ10 was one order of magnitude lower in succinate cytochrome c reductase than in NADH cytochrome c reductase. The Km value in NADH oxidation approaches the natural CoQ content of beef heart mitochondria, whereas that in succinate oxidation is close to the content of respiratory chain enzymes.[Abstract] [Full Text] [Related] [New Search]