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  • Title: [Method of simultaneous determination of kallikrein, plasmin and thrombin precursors and inhibitors in human blood plasma].
    Author: Gomazkov OA, Komissarova NV.
    Journal: Biull Eksp Biol Med; 1976 May; 81(5):632-4. PubMed ID: 132976.
    Abstract:
    Prekallikrein, plasminogen and prothrombin of human blood plasma have been separately activated by caolin streptokinase and thromboplastin. By measuring the TAME-esterase (N-d Tozy-L-arginine methyl ester) activity of each enzyme and its changes in the course of plasma incubation with the activator, it was possible to estimate the values of precursors of kallikrein, plasmin, thrombin and their inhibitors. Evidence is given that under conditions described the activation is specific of each enzyme and does not affect the level of the two other percursors. The method has been developed in two modifications, permitting to obtain the value of seven parameters in 0.4--0.7 ml of blood plasma.
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