These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Calcium control of actin-activated myosin adenosine triphosphatase from Dictyostelium discoideum.
    Author: Mockrin SC, Spudich JA.
    Journal: Proc Natl Acad Sci U S A; 1976 Jul; 73(7):2321-5. PubMed ID: 133352.
    Abstract:
    A protein fraction from the cellular slime mold Dictyostelium discoideum confers Ca2+-sensitivity on the activation of purified myosin adenosinetriphosphatase (ATP phosphohydrolase, EC 3.6.1.3) from Dictyostelium by purified Dictyostelium actin. That is, the fraction inhibits the actomyosin adenosine triphosphatase activity in the absence of Ca+ but not in the presence of Ca2+. This Ca2+-sensitizing factor affects only the actin-activated myosin adenosine triphosphatase and not the enzyme activity of myosin alone. The Ca2+-sensitivity is conserved when muscle actin replaces Dictyostelium actin, but is lost when muscle myosin replaces Dictyostelium myosin. The factor appears to be a protein since it is nondialyzable, is heat labile, and can be precipitated with ammonium sulfate. The factor can be purified 70-fold on an actin-affinity column.
    [Abstract] [Full Text] [Related] [New Search]